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浮游菌采樣器采樣前的準備工作及步驟</div> <div id="i0amume" class="inside_rnewscon"> <h1 class="newsname">浮游菌采樣器采樣前的準備工作及步驟</h1> <li id="2auomek" class="time" style="padding:15px 0; text-align:center; color:#b4b4b4;">更新日期：2021-10-22 信息來(lái)源：瀏覽次數：1069</li> <ul class="dcont"> <p style="text-align: justify;"> <strong>浮游菌采樣器</strong>是一種的多孔吸入式塵菌采樣器。其原理是，根據顆粒撞擊原理和等速采樣理論設計，采樣直接，采樣口風(fēng)速與潔凈室內風(fēng)速基本一致，能更準確地反映潔凈室內的微生物濃度。<div>　　采樣時(shí)，帶塵菌空氣高速通過(guò)微孔，被撞擊在培養皿內的瓊脂表面;這些活體微生物在培養過(guò)程中，發(fā)生動(dòng)態(tài)再水化過(guò)程，高速生長(cháng)，從而更快得出結果。</div><div>　　一、浮游菌采樣器采樣前的準備工作</div><div>　　1、被測試手術(shù)室(潔凈室)溫、濕度須達到規定的要求;靜壓差、換氣次數、空氣流速必須控制在規定值內。</div><div>　　2、對單向流測試應在凈化空調系統正常運行不少于10min;非單向流不少于30min后開(kāi)始。</div><div>　　3、靜態(tài)測試時(shí),室內人員不多于2人。采樣時(shí)測試人員應在采樣口下風(fēng)側。</div><div>　　4、測試人員應按無(wú)菌操作規程進(jìn)行。采取一切措施防止采樣口的污染和其它人為對樣本的污染。</div><div>　　5、測試人員須穿戴符合潔凈級別的潔凈服。</div><div>　　6、浮游菌測試前,被測手術(shù)室(潔凈室)已經(jīng)過(guò)消毒。</div><div>　　二、浮游菌采樣器的采樣步驟</div><div>　　1、在有平臺的潔凈室內編制培養皿,操作前應用浸濕75%乙醇的醫用脫脂棉花擦拭平臺。測試人員雙手用同樣方法嚴格消毒。在編制過(guò)程中再次仔細檢查培養基平皿的質(zhì)量,有變質(zhì)、破損或污染不能使用。</div><div>　　2、用浸濕75%乙醇的醫用脫脂棉花擦拭采樣器的采樣口,采樣管、采樣器的項蓋,轉盤(pán)及罩子內外表面。</div><div>　　3、采樣器經(jīng)消毒后不放入培養皿,啟動(dòng)儀器空轉運行不少于5min,使儀器中的殘余的毒劑劑蒸發(fā)。</div><div>　　4、根據手術(shù)室的潔凈度級別設定采樣量，采樣器的流量(L/min)X采樣時(shí)間(min)=采樣量(L),每次小的采樣量見(jiàn)表。采樣器的流量</div><div>　　5、放入培養皿,蓋上蓋子后調節采樣器縫隙高度。采樣操作時(shí),培養皿應輕拿輕放,用“五指抓拿”培養皿外徑,盡量避免人為對樣本造成污染。</div><div>　　6、采樣點(diǎn)的布置及采樣點(diǎn)數量。</div></p> 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